Journal: Molecular cancer research : MCR

Article Title: Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues

doi: 10.1158/1541-7786.MCR-16-0262

Figure Lengend Snippet: Isogenic cell lines. A, Schematic overview of the protocol used to prepare frozen and FFPE cell samples. The steps are discussed in Supplementary Methods. B, Venn diagram showing the intersection between frozen and FFPE metabolomic data in the experimental settings. C, Box-and-whisker plot representing the relative signal intensity of all shared metabolites found in frozen and FFPE samples. D, Bar plot of the metabolite number found in frozen and FFPE samples. The metabolites are categorized according to the class membership. The percentage above each bar represents the number of detectable metabolites (of each class) found in FFPE compared with frozen samples. E, Correlation plots between FFPE cell replicates and between frozen and FFPE cell samples. F, Box-and-whisker plots of the correlation coefficients, categorized to the class membership, between frozen replicates, FFPE replicates, and frozen and FFPE samples. G, Heatmap of selected metabolites from cell line samples. Hierarchical clustering (Ward method) based on KODAMA dissimilarity matrix is used for unsupervised classification. The phenotypic labels of the samples (i.e., LNCaP and LNCaP-Abl) are indicated as a colored band on top of the heatmap.

Article Snippet: Metabolite profiling Metabolite profiling was conducted by the company Metabolon Inc. as previously described by Evans and colleagues ( 13 ).

Techniques: Whisker Assay

Journal: Molecular cancer research : MCR

Article Title: Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues

doi: 10.1158/1541-7786.MCR-16-0262

Figure Lengend Snippet: Human prostate. A, Schematic diagram of the human samples used. B, Venn diagram showing the intersection between frozen and FFPE metabolomic data in the experimental settings. C, Bar plot of the metabolite number found in frozen and FFPE samples. The metabolites are categorized according to the class membership. The percentage above each bar represents the number of detectable metabolites (for each class) found in FFPE compared with frozen samples. D, Correlation plots between FFPE cell replicates and between frozen and FFPE cell samples. E, Heatmap of selected metabolites from cell line samples. Hierarchical clustering (Ward method) based on KODAMA dissimilarity matrix is used for unsupervised/semisupervised classification. The phenotypic labels of the samples (i.e., normal and tumor tissue) are indicated as a colored band on top of the heatmap.

Article Snippet: Metabolite profiling Metabolite profiling was conducted by the company Metabolon Inc. as previously described by Evans and colleagues ( 13 ).

Techniques:

Journal: Nutrients

Article Title: NMR-Based Multi Parametric Quality Control of Fruit Juices: SGF Profiling

doi: 10.3390/nu1020148

Figure Lengend Snippet: SGF Profiling quantification results of an orange juice. The colored flags show agreements with or deviations from reference intervals, which are provided by the European Fruit Juice Association A.I.J.N. (N/Q: Not quantified due to non-detectable signal or insufficient signal assignment).

Article Snippet: One example of the successful application of this technique is the Bruker JuiceScreener™ for SGF Profiling™ [ , ].

Techniques: